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In the south-western region of Australia, allometric relationships between tree dimensional measurements and total tree biomass were developed for estimating carbon sequestered in native eucalypt woodlands. A total of 71 trees representing eight local native species from three genera were destructively sampled. Within this sample set, below ground measurements were included for 51 trees, enabling the development of allometric equations for total biomass applicable to small, medium, and large native trees.Adiversity of tree dimensions were recorded and regressed against biomass, including stem diameter at 130 cm (DBH), stem diameter at ground level, stem diameter at 10 cm, stem diameter at 30 cm, total tree height, height of canopy break and meancanopy diameter.DBHwas consistently highly correlated with above ground, below ground and total biomass. However, measurements of stem diameters at 0, 10 and 30 cm, and mean canopy diameter often displayed equivalent and at times greater correlation with tree biomass. Multi-species allometric equations were also developed, including ‘Mallee growth form’ and ‘all-eucalypt’ regressions. These equations were then applied to field inventory data collected from three locally dominant woodland types and eucalypt dominated environmental plantings to create robust relationships between biomass and stand basal area. This study contributes the predictive equations required to accurately quantify the carbon sequestered in native woodland ecosystems in the low rainfall region of south-western Australia.